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easysep® human whole blood cd3 positive selection kit  (STEMCELL Technologies Inc)

 
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    Structured Review

    STEMCELL Technologies Inc easysep® human whole blood cd3 positive selection kit
    Impact of whole blood and selected <t>CD3</t> + cell chimerism on overall survival (OS) and relapse free survival (RFS) at day +30(D30) and day +90 (D90). Graft survival analysis were death censored. Statistical analyzes about conditioning with anti‐thymocyte globulin (ATG) have not been done ( n = 6). (A) Statistical analysis with a full donor chimerism >95%. Statistical analysis with a full donor chimerism >99%
    Easysep® Human Whole Blood Cd3 Positive Selection Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easysep%C2%AE+human+whole+blood+cd3+positive+selection+kit/easysep+human+t+cell+isolation+kit/pmc09303291-56-13-21
    Average 90 stars, based on 1 article reviews
    easysep® human whole blood cd3 positive selection kit - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Utility of assessing CD3 + cell chimerism within the first months after allogeneic hematopoietic stem‐cell transplantation for acute myeloid leukemia"

    Article Title: Utility of assessing CD3 + cell chimerism within the first months after allogeneic hematopoietic stem‐cell transplantation for acute myeloid leukemia

    Journal: Hla

    doi: 10.1111/tan.14557

    Impact of whole blood and selected CD3 + cell chimerism on overall survival (OS) and relapse free survival (RFS) at day +30(D30) and day +90 (D90). Graft survival analysis were death censored. Statistical analyzes about conditioning with anti‐thymocyte globulin (ATG) have not been done ( n = 6). (A) Statistical analysis with a full donor chimerism >95%. Statistical analysis with a full donor chimerism >99%
    Figure Legend Snippet: Impact of whole blood and selected CD3 + cell chimerism on overall survival (OS) and relapse free survival (RFS) at day +30(D30) and day +90 (D90). Graft survival analysis were death censored. Statistical analyzes about conditioning with anti‐thymocyte globulin (ATG) have not been done ( n = 6). (A) Statistical analysis with a full donor chimerism >95%. Statistical analysis with a full donor chimerism >99%

    Techniques Used:

    Related Articles

    Isolation:

    Article Title: Assessment of the purity of isolated cell populations for lineage-specific chimerism monitoring post haematopoietic stem cell transplantation.
    Article Snippet: Following haematopoietic stem cell transplantation, monitoring the proportion of donor and recipient haematopoiesis in the patient (chimerism) is an influential tool in directing further treatment choices.. Short tandem repeat (STR) analysis is a method of chimerism monitoring using DNA isolated from peripheral blood, bone marrow or specific isolated cell lineages such as CD3+ T cells.. For lineage-specific STR analysis on cell populations isolated from peripheral blood, a qualitative estimation of the purity of each isolated population is essential for the correct interpretation of the test data.

    Article Title: A one-step assay for sorted CD3 + cell purity and chimerism after hematopoietic stem cell transplantation.
    Article Snippet: A hematopoietic chimerism assay is the laboratory test for monitoring engraftment and quantifying the proportions of donor and recipient cells after hematopoietic stem cell transplantation recipients.. Flow cytometry is the reference method for determining the purity of CD3+ cells on the chimerism of selected CD3+ cells.. In the present study, we developed a single-step procedure that combines the CD3+ purity assay (using the PCR-based Non-T Genomic Detection Kit from Accumol, Calgary, Canada) and the qPCR chimerism monitoring assay (the QTRACE® qPCR assay from Jeta Molecular, Utrecht, the Netherlands).

    Article Title: Utility of assessing CD3 + cell chimerism within the first months after allogeneic hematopoietic stem‐cell transplantation for acute myeloid leukemia
    Article Snippet: CD3 + cells were isolated from 7 ml peripheral blood samples using the EasySep® Human Whole Blood CD3 Positive Selection Kit (STEMCELL Technologies, Vancouver, BC, Canada).

    Selection:

    Article Title: Assessment of the purity of isolated cell populations for lineage-specific chimerism monitoring post haematopoietic stem cell transplantation.
    Article Snippet: Following haematopoietic stem cell transplantation, monitoring the proportion of donor and recipient haematopoiesis in the patient (chimerism) is an influential tool in directing further treatment choices.. Short tandem repeat (STR) analysis is a method of chimerism monitoring using DNA isolated from peripheral blood, bone marrow or specific isolated cell lineages such as CD3+ T cells.. For lineage-specific STR analysis on cell populations isolated from peripheral blood, a qualitative estimation of the purity of each isolated population is essential for the correct interpretation of the test data.

    Article Title: A one-step assay for sorted CD3 + cell purity and chimerism after hematopoietic stem cell transplantation.
    Article Snippet: A hematopoietic chimerism assay is the laboratory test for monitoring engraftment and quantifying the proportions of donor and recipient cells after hematopoietic stem cell transplantation recipients.. Flow cytometry is the reference method for determining the purity of CD3+ cells on the chimerism of selected CD3+ cells.. In the present study, we developed a single-step procedure that combines the CD3+ purity assay (using the PCR-based Non-T Genomic Detection Kit from Accumol, Calgary, Canada) and the qPCR chimerism monitoring assay (the QTRACE® qPCR assay from Jeta Molecular, Utrecht, the Netherlands).

    Article Title: Utility of assessing CD3 + cell chimerism within the first months after allogeneic hematopoietic stem‐cell transplantation for acute myeloid leukemia
    Article Snippet: CD3 + cells were isolated from 7 ml peripheral blood samples using the EasySep® Human Whole Blood CD3 Positive Selection Kit (STEMCELL Technologies, Vancouver, BC, Canada).

    Flow Cytometry:

    Article Title: Assessment of the purity of isolated cell populations for lineage-specific chimerism monitoring post haematopoietic stem cell transplantation.
    Article Snippet: Following haematopoietic stem cell transplantation, monitoring the proportion of donor and recipient haematopoiesis in the patient (chimerism) is an influential tool in directing further treatment choices.. Short tandem repeat (STR) analysis is a method of chimerism monitoring using DNA isolated from peripheral blood, bone marrow or specific isolated cell lineages such as CD3+ T cells.. For lineage-specific STR analysis on cell populations isolated from peripheral blood, a qualitative estimation of the purity of each isolated population is essential for the correct interpretation of the test data.

    Article Title: A one-step assay for sorted CD3 + cell purity and chimerism after hematopoietic stem cell transplantation.
    Article Snippet: A hematopoietic chimerism assay is the laboratory test for monitoring engraftment and quantifying the proportions of donor and recipient cells after hematopoietic stem cell transplantation recipients.. Flow cytometry is the reference method for determining the purity of CD3+ cells on the chimerism of selected CD3+ cells.. In the present study, we developed a single-step procedure that combines the CD3+ purity assay (using the PCR-based Non-T Genomic Detection Kit from Accumol, Calgary, Canada) and the qPCR chimerism monitoring assay (the QTRACE® qPCR assay from Jeta Molecular, Utrecht, the Netherlands).

    Article Title: Utility of assessing CD3 + cell chimerism within the first months after allogeneic hematopoietic stem‐cell transplantation for acute myeloid leukemia
    Article Snippet: CD3 + cells were isolated from 7 ml peripheral blood samples using the EasySep® Human Whole Blood CD3 Positive Selection Kit (STEMCELL Technologies, Vancouver, BC, Canada).



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    easysep® human whole blood cd3 positive selection kit  (STEMCELL Technologies Inc)
    90
    STEMCELL Technologies Inc easysep® human whole blood cd3 positive selection kit
    Impact of whole blood and selected <t>CD3</t> + cell chimerism on overall survival (OS) and relapse free survival (RFS) at day +30(D30) and day +90 (D90). Graft survival analysis were death censored. Statistical analyzes about conditioning with anti‐thymocyte globulin (ATG) have not been done ( n = 6). (A) Statistical analysis with a full donor chimerism >95%. Statistical analysis with a full donor chimerism >99%
    Easysep® Human Whole Blood Cd3 Positive Selection Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easysep%C2%AE+human+whole+blood+cd3+positive+selection+kit/easysep+human+t+cell+isolation+kit/pmc09303291-56-13-21
    Average 90 stars, based on 1 article reviews
    easysep® human whole blood cd3 positive selection kit - by Bioz Stars, 2026-09
    90/100 stars
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    easysep human whole blood cd3 positive selection kit  (STEMCELL Technologies Inc)
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    STEMCELL Technologies Inc easysep human whole blood cd3 positive selection kit
    (A) Baseline number of foci per nucleus as a function of age in <t>CD3</t> + lymphocytes extracted from finger prick collection in 339 healthy donors (***p < 0.0001, deviation from zero of the slope of linear regression). Four donors in this cohort were considered healthy but were in remission from cancer at the time of the finger prick (shown as red dots). See also . (B) Distribution of spontaneous foci per nucleus after bead-based extraction of CD3 + lymphocytes from finger prick samples (finger prick), Ficoll-based extraction of PBMCs from buffy coat samples (PBMC baseline), and a further freeze-thaw cycle and plating in the cell culture media (PBMC 0 Gy). ***p < 0.0001, one-way ANOVA, Tukey’s post hoc test for multiple comparisons among all three groups: 1–2, 2–3, and 1–3. Boxplots show median ± quartiles ± min/max values.
    Easysep Human Whole Blood Cd3 Positive Selection Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easysep%C2%AE+human+whole+blood+cd3+positive+selection+kit/easysep+human+t+cell+isolation+kit/pmc07784531-274-25-33
    Average 90 stars, based on 1 article reviews
    easysep human whole blood cd3 positive selection kit - by Bioz Stars, 2026-09
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    isolated pb cd3þ t cells easysep human whole blood cd3 positive selection kit  (STEMCELL Technologies Inc)
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    STEMCELL Technologies Inc isolated pb cd3þ t cells easysep human whole blood cd3 positive selection kit
    (A) Baseline number of foci per nucleus as a function of age in <t>CD3</t> + lymphocytes extracted from finger prick collection in 339 healthy donors (***p < 0.0001, deviation from zero of the slope of linear regression). Four donors in this cohort were considered healthy but were in remission from cancer at the time of the finger prick (shown as red dots). See also . (B) Distribution of spontaneous foci per nucleus after bead-based extraction of CD3 + lymphocytes from finger prick samples (finger prick), Ficoll-based extraction of PBMCs from buffy coat samples (PBMC baseline), and a further freeze-thaw cycle and plating in the cell culture media (PBMC 0 Gy). ***p < 0.0001, one-way ANOVA, Tukey’s post hoc test for multiple comparisons among all three groups: 1–2, 2–3, and 1–3. Boxplots show median ± quartiles ± min/max values.
    Isolated Pb Cd3þ T Cells Easysep Human Whole Blood Cd3 Positive Selection Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Impact of whole blood and selected CD3 + cell chimerism on overall survival (OS) and relapse free survival (RFS) at day +30(D30) and day +90 (D90). Graft survival analysis were death censored. Statistical analyzes about conditioning with anti‐thymocyte globulin (ATG) have not been done ( n = 6). (A) Statistical analysis with a full donor chimerism >95%. Statistical analysis with a full donor chimerism >99%

    Journal: Hla

    Article Title: Utility of assessing CD3 + cell chimerism within the first months after allogeneic hematopoietic stem‐cell transplantation for acute myeloid leukemia

    doi: 10.1111/tan.14557

    Figure Lengend Snippet: Impact of whole blood and selected CD3 + cell chimerism on overall survival (OS) and relapse free survival (RFS) at day +30(D30) and day +90 (D90). Graft survival analysis were death censored. Statistical analyzes about conditioning with anti‐thymocyte globulin (ATG) have not been done ( n = 6). (A) Statistical analysis with a full donor chimerism >95%. Statistical analysis with a full donor chimerism >99%

    Article Snippet: CD3 + cells were isolated from 7 ml peripheral blood samples using the EasySep® Human Whole Blood CD3 Positive Selection Kit (STEMCELL Technologies, Vancouver, BC, Canada).

    Techniques:

    (A) Baseline number of foci per nucleus as a function of age in CD3 + lymphocytes extracted from finger prick collection in 339 healthy donors (***p < 0.0001, deviation from zero of the slope of linear regression). Four donors in this cohort were considered healthy but were in remission from cancer at the time of the finger prick (shown as red dots). See also . (B) Distribution of spontaneous foci per nucleus after bead-based extraction of CD3 + lymphocytes from finger prick samples (finger prick), Ficoll-based extraction of PBMCs from buffy coat samples (PBMC baseline), and a further freeze-thaw cycle and plating in the cell culture media (PBMC 0 Gy). ***p < 0.0001, one-way ANOVA, Tukey’s post hoc test for multiple comparisons among all three groups: 1–2, 2–3, and 1–3. Boxplots show median ± quartiles ± min/max values.

    Journal: Cell reports

    Article Title: DNA Damage Baseline Predicts Resilience to Space Radiation and Radiotherapy

    doi: 10.1016/j.celrep.2020.108434

    Figure Lengend Snippet: (A) Baseline number of foci per nucleus as a function of age in CD3 + lymphocytes extracted from finger prick collection in 339 healthy donors (***p < 0.0001, deviation from zero of the slope of linear regression). Four donors in this cohort were considered healthy but were in remission from cancer at the time of the finger prick (shown as red dots). See also . (B) Distribution of spontaneous foci per nucleus after bead-based extraction of CD3 + lymphocytes from finger prick samples (finger prick), Ficoll-based extraction of PBMCs from buffy coat samples (PBMC baseline), and a further freeze-thaw cycle and plating in the cell culture media (PBMC 0 Gy). ***p < 0.0001, one-way ANOVA, Tukey’s post hoc test for multiple comparisons among all three groups: 1–2, 2–3, and 1–3. Boxplots show median ± quartiles ± min/max values.

    Article Snippet: CD3+ lymphocytes were isolated from fixed blood samples - collected via finger prick for healthy donors and blood draw for cancer patients - using the EasySep Human Whole Blood CD3 Positive Selection Kit (StemCell Tech Cat# 18081).

    Techniques: Extraction, Cell Culture

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: DNA Damage Baseline Predicts Resilience to Space Radiation and Radiotherapy

    doi: 10.1016/j.celrep.2020.108434

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: CD3+ lymphocytes were isolated from fixed blood samples - collected via finger prick for healthy donors and blood draw for cancer patients - using the EasySep Human Whole Blood CD3 Positive Selection Kit (StemCell Tech Cat# 18081).

    Techniques: Recombinant, Red Blood Cell Lysis, Selection, Flow Cytometry, Multiplex Assay, Software